Protein Bioengineering Laboratory

The Protein Bioengineering Laboratory focuses on new technologies in the fields of biochemistry, biophysics, and molecular biology with the aim of developing innovative biomaterials for biotechnological applications. The Laboratory specialises in conducting interdisciplinary research that can entail work in the areas of genetic engineering, biochemistry and biophysics. The research focuses on analysing the functions of proteins and peptides, as well as protein-to-protein and protein-to-ligand interactions. The Laboratory takes advantage of laboratory space furnished with lab furniture and fume cupboards as well as a number of adjacent rooms that can be adapted to the needs at hand.

The laboratory performs lab work for scientific and commercial research projects. Its offer includes:
- development/design of expressive systems for the purposes of producing recombinant proteins,
- purification of native and recombinant proteins from various biological systems,
- analyses of intercellular interactions and assessment of the interaction kinetics parameters.

Laboratory equipment:

1. FPLC setups for chromatographic purification of peptides, proteins and protein complexes:
- liquid chromatography device for pilot-plant protein purification, AKTA PILOT GE Healthcare Life Sciences,
- liquid chromatography device for laboratory applications with the throughput up to 150 ml/min: AKTA AVANT GE Healthcare Life Sciences,
- liquid chromatography device for laboratory applications with the throughput up to 50 ml/min: AKTA Prime Plus GE Healthcare Life Sciences,
- liquid chromatography device for laboratory applications: AKTA Pure GE Healthcare Life Sciences.

2. Instrument setups for analysing intercellular interactions and assessing interaction kinetics (Kd, kon, koff):
- device for measuring interactions by means of bio-layer interferometry (BLI): OctetRED 96 (ForteBIO),
- device for measuring interaction by means of microscale thermophoresis (MST): Monolith NT.115Pico and NT.LabelFree (Nanotemper),
- device for temperature gradient monitoring of structural changes in protein molecules
- Prometheus NT.48 (Nanotemper).

3. Additional equipment:
- automatic multilabel microplate reader Victor X4 (Perkin Elmer),
- flow cytometer FACSVerse (BD)


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