Laboratory of Genomics, Transcriptomics and Proteomics

The genomics, transcriptomics and proteomics lab comprises the following key work stations:

1.     Research material isolation station.

Tasks performed: Isolation of DNA, RNA, short-chain RNA (including miRNA) and protein from various types of biological material, e.g. cells, blood samples, tissue specimens, or entire organisms.

2.     Research material evaluation station.

Tasks: Qualitative and quantitative assessment of the isolated research material, i.e. DNA, RNA, short-chain RNA (including miRNA), oligonucleotides, and protein.

Key equipment:

Qubit3.0 Fluorometer

Agilent 2100 Bioanalyzer

3.     Gene expression station.

Tasks: Studies of gene and miRNA expression using the real-time PCR method with OpenArray compatibility.

Key equipment:

Applied BiosystemsQuantStudio12K Flex Real-Time PCR System

QuantStudio12K Flex AccuFillSystem

QuantStudio3D Digital PCR System

Twister® Automation Robot

4.     Flow cytometry station.

Tasks: Analyses of microcell sub-populations in human blood.

Key equipment:

Apogee Micro Flow Cytometer

5.     Liquid biopsy station.

Tasks: Isolation of circulating tumour cells, leukocytes and extracellular DNA from the blood of patients diagnosed with breast, ovarian or pulmonary cancer.

Analysis of the isolated material with the view of early detection, prognostics and monitoring of the neoplastic disease, as well as assessing treatment effects.

Key equipment:

LiquidBiopsy® Automated Rare Cell Isolation Platform

6.     Sequencing station.

Tasks: high-throughput genetic sequencing (DNA, RNA, miRNA) with the use of NSG (next generation sequencing) technology.

Key equipment:

Ion S5XL System

Ion ChefSystem

Ion Personal Genome Machine™ System